Son of a school teacher and musician, Kirkpatrick grew up in a musical atmosphere. In 1854, he went to Philadelphia, Pennsylvania, to study music and learn a trade; he spent over three years as a carpenter. But he was more interested in music than mechanics, devoting all his leisure time to its study. His ambition at the time was to become a violinist.

In 1855, Kirkpatrick joined the Wharton Street Methodist Episcopal Church in Philadelphia, and from then on devoted himself mostly to sacred music, giving his services to the choir and Sunday school. As there were few church organs in that day, his violin and cello were in constant demand for choir rehearsals, singing societies, and church programs. During this time he wrote a number of unpublished hymn tunes and anthems.

Kirkpatrick studied vocal music under Professor T. Bishop, then a leading oratorio and ballad singer, and became a member of the Harmonia and Handel and Haydn Sacred Music Societies, where he heard the greatest singers of the day and became familiar with the principal choral works of the great composers. Kirkpatrick's first published composition was "When the Spark of Life Is Waning," which appeared around 1858 in the Musical Pioneer in New York. He went on to publish about 50 hymn collections, many in collaboration with John Sweney. His works include:

• The Wells of Salvation: Songs for the Sabbath School (Philadelphia, Pennsylvania: John J. Hood, 1881) (with John Sweney)
• Winning Songs (Philadelphia, Pennsylvania: John J. Hood, 1892) (with John Sweney & Henry Gilmour)
• Songs of Love and Praise, No. 3 (Philadelphia, Pennsylvania: John J. Hood, 1896) (with John Sweney & Henry Gilmour)
• Glorious Praise, with Howard Doane (Louisville, Kentucky: The Baptist Book Concern, 1904)
• Revival Melodies, 1921 (editor)
• Waves of Glory No. 2, 1921 (editor)

Melodies for:

Away in a Manger (one tune)

The Comforter Is Come
He Hideth My Soul
Jesus Saves!
Lead Me to Calvary
Lord I'm Coming Home (Also lyrics)
O To Be Like Thee
Redeemed
Stepping in the Light
'Tis So Sweet to Trust in Jesus
We Have an Anchor
You May Have the Joy Bells